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Main human basophils and BDCA1+ DCs were being isolated from whole-blood donations. Human peripheral total-blood was attained from volunteering donors, who offered knowledgeable consent in accordance with the Helsinki Declaration. The study was authorised because of the local ethics committee (KEK 2018-00204). Basophils and BDCA1+ DCs had been enriched by Percoll density centrifugation of dextran-sedimented supernatants.
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CD23 interacts with IgE-Fc in the hinge region in between Cε3 and Cε4 domains (Fig. 2j), favoring a closed conformation of Cε3 domains34. Comparison of the CD23 and ligelizumab interaction web sites on IgE indicates fairly minimal overlap concerning binding web sites of both of these IgE ligands (Fig. 2k). In addition, the orientation on the ligelizumab VHVL domains on IgE (Fig. 2g) implies the ligelizumab Fab would job away from the IgE and wouldn't sterically overlap certain CD23, suggesting that Competitiveness for IgE surface area subsites and the stabilization of an open Cεthree conformation would be the first mechanism of CD23 inhibition.